Journal: BMC Microbiology

Article Title: A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities

doi: 10.1186/1471-2180-8-195

Figure Lengend Snippet: Short oligonucleotide melting curve profile of the perfect, 1 nt and 2 nt mismatch probes . Melting curve profile of the perfect match probe BLON135a (◆), 1 nt mismatch probe BLON135b (■) and 2 nt mismatch probe BLON135c (●) after hybridisation with fluorescently labelled 16S ribosomal DNA PCR products from Bifidobacterium longum . The error bars represent the standard deviation.

Article Snippet: The initial DNA denaturation step was performed at 96°C for 4 min using a PCR sprint thermocycler (Hybaid, UK), followed by 15 cycles of 96°C for 1 min, 53°C for 1 min, and 72°C for 1 min 10 s. A final step of 72°C for 4 min completed the reaction.

Techniques: Hybridization, Standard Deviation

Journal: BMC Microbiology

Article Title: A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities

doi: 10.1186/1471-2180-8-195

Figure Lengend Snippet: Faecal bacterial community analysis using DGGE . (A) DGGE analysis, in a denaturing gradient of 40–60% urea and formamide, of the PCR products from the amplification of faecal bacterial genomic DNA from three healthy individuals (A, B and C) and a UC patient in active (D) and remission (R) states of the disease, using primers for 16S ribosomal gene region V6-V8. (B) DGGE analysis, in a denaturing gradient of 30–60% urea and formamide, of the PCR products from the amplification of faecal bacterial genomic DNA from three healthy individuals (A, B and C) and a UC patient in active (D) and remission (R) states of the disease, using primers for 16S rDNA region V3. For both gels, numbered arrows point to bands subjected to re-amplification and sequencing, and correspond to the amplicon identities in Table (V6-V8) and Table (V3). The ladder on each gel is used for quality control purposes only.

Article Snippet: The initial DNA denaturation step was performed at 96°C for 4 min using a PCR sprint thermocycler (Hybaid, UK), followed by 15 cycles of 96°C for 1 min, 53°C for 1 min, and 72°C for 1 min 10 s. A final step of 72°C for 4 min completed the reaction.

Techniques: Amplification, Sequencing, Control